Kinetic data were fit to the Michaelis-Menten equation with the MicroCal software by using the Levenberg-Marquard algorithm. Degrees – All Downloads. Moreover, the presence of a divalent metal, most likely iron, as deduced from the crystal structure of PfcPGI bound with 6-phosphogluconate is a prerequisite in the recently proposed hydride transfer mechanism of PfcPGI The metal content measured depended on both the source of the protein and the purification procedure used due to different contaminating metal contents of the buffer systems used. Temperature optimum and thermostability.
All V max values given correspond to the purification procedure described. National Center for Biotechnology Information , U. The deduced activation energy of the latter was significant lower than the corresponding values of the other cPGIs in the present study. Crystal structure of rabbit phosphoglucose isomerase, a glycolytic enzyme that moonlights as neuroleukin, autocrine motility factor, and differentiation mediator. Workshop on “Scientific Writing” – February.
Postgraduate Institute of Science
In sedimentation velocity experiments the apparent sedimentation coefficient s app 20,W and an approximated molecular mass were evaluated from the velocity and shape of the sedimenting boundary by fitting the time-dependent concentration profiles calculated with Lamm’s differential equation for a single sedimenting species to the measured data by using the program package AKKUPROG Glucosephosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: Phylogenetic analyses and evolution of cPGIs.
Sequence comparison suggested the presence fhesis few putative cPGI homologs; however, none of them have been characterized 44 Sc in Applied Statistics. The reference or source is indicated in parentheses in the subheading.
In order to obtain metal-free enzyme, 2 mg of enzyme was incubated in the presence of 0. Kinetic data were fit to the Michaelis-Menten equation with the MicroCal software by using the Levenberg-Marquard algorithm.
Pgis thesis format outline – College Students Essay
National Center for Biotechnology InformationU. Temperature dependence and thermal stability. Crystal structure of rabbit phosphoglucose isomerase, a glycolytic enzyme that moonlights as neuroleukin, autocrine motility factor, and differentiation mediator.
The vials were then cooled on ice for 5 min, and the residual enzyme activity was tested and compared to the controls unheated samples. Determination of iron and nickel was performed by atom absorption spectroscopy as described recently It should be noted that S. It has laid a solid foundation for my future studies. The metal content measured depended on both the source of the protein and the purification procedure used due to different contaminating metal contents of the buffer systems used.
Structure of native phosphoglucose isomerase from rabbit: New Year Celebrations – Structure of oxalate decarboxylase from Bacillus subtilis at 1.
Heat-induced unfolding of the respective cPGIs was analyzed in temperature gradient experiments by using closed cuvettes 0. PGI is also involved in gluconeogenesis, where the enzyme operates in the reverse direction see references 2226and Janani de Silva M.
Pgis thesis format outline
Phosphoglucose isomerase from Escherichia coli K Tyr, Ala69, and Ala, which have been suggested to contribute to the hydrophobicity of the active site of PfcPGI, are not conserved.
Cloning, characterization, and nucleotide sequence analysis of a Zymomonas mobilis phosphoglucose isomerase gene that is subject to carbon source-dependent regulation.
tthesis The X-ray crystal structure of phosphomannose isomerase from Candida albicans at 1. The measured K Me,0. Abstract Cupin-type phosphoglucose isomerases cPGIs were identified in some archaeal and bacterial genomes and the respective coding function of cpgi ‘s from the euryarchaeota Archaeoglobus fulgidus and Methanosarcina mazeias well as the bacteria Salmonella enterica serovar Typhimurium and Ensifer melilotiwas proven by functional overexpression.
Evolution of functional diversity in the cupin superfamily.
The measured concentration profile of sedimentation-diffusion equilibrium runs were best fit by using a molecular mass of 27 kDa, which is about the calculated mass of the AfcPGI De Vos, and O. When the measured concentration profile of sedimentation-diffusion equilibrium runs remained unchanged for 12 h, we assumed equilibrium to be attained.
Temperature optimum and thermostability. Effect of metals on PGI activity. All metals were used in the form of chlorides.